[Protein-analysis] higher bands than expected

FAROOQ SABAR farooqsabar at yahoo.com
Tue May 9 02:40:15 EST 2006


Hello!
I purified my protein and analyzed on SDS-PAGE. There
was only a single band of required size which is about
19kda. I stored the protein solution at 4degrees C and
now after three months when I run the protein on 8%
SDS-PAGE there are multiple bands. All bands are above
the required size except a line like band at the dye
front. Note that I am using three months old stock of
gel running buffer and SDS solution. Is there some
thing wrong with the protein or gel or loading buffer?
Can some one guide me? Thank you.

Muhammad Farooq Sabar


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