[Protein-analysis] IEF transfer
hostetle at hotmail.com
Wed May 10 08:59:16 EST 2006
I saw your comment on an IEF transfer problem, and I was hoping you could
First, any help you can offer would be appreciated, but if you are unable,
thank you anyway.
To my point, I am trying to do an IEF slab gel and I have tried both
nondenaturing and denaturing conditions in a pH range of 3-7. After I do my
transfer I silver stain the gel and I have found a great deal of proteins
still on the gel. I have tried to transfer in Acetic Acid I have tried
transfering with 192mM Glycine, 50mM Tris, 0,1%SDS, 20%Methanol, but still
not all the proteins are transfering. The only proteins that show up in
Ponceau are those near the cathodic end. Do you have any suggestions?
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