[Protein-analysis] why I can see my band dyed in proceau S on PDFC?

=?GB2312?B?veDV1A==?= via proteins%40net.bio.net (by janejiesweet At gmail.com)
Fri Nov 24 21:57:51 EST 2006


sir/madam:
     I have been trying to blot proteins of brain from an 8% SDS PAGE
toBioRad PVDF membrane. I thought that my blot was unsuccessful
afterattempting to visualize by Ponceau S. I could not even detect my
MWstandards.but the same process worked well in NCmembrane,where the band
showed clearly.. Any clues as to why my ponceau S did not work? I diluted a
fresh Sigam concentrate (20 mL) into 180 mL ofdeionized water. I stained for
5 min and watch as the membrane destained in 1% AcOH. At no time did I see
anything resembling a protein band. This problem has occured for the past
two consecutive times that I have tried the technique. Your comments are
appreciated.


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