[Protein-analysis] How to deal with proteins precipitating in an IEF gel?

[Garcia, Rafael]

After focusing my proteins on an IEF gel, there are a number of clearly
visible white bands, especially at the "+" end.  I assume that this is
protein precipitating at it's isoelectric point.  How do I get these
back into solution to run the 2nd dimension?  Any tricks?

 

Rafael Garcia
Lead Scientist
USDA-ARS
Eastern Regional Research Center
600 East Mermaid Lane
Wyndmoor PA 19038

Rafael.Garcia from ars.usda.gov
voice: 215-836-3743
fax: 215-233-6795