[Protein-analysis] How to deal with proteins precipitating in an IEF gel?

Garcia, Rafael via proteins%40net.bio.net (by Rafael.Garcia from ARS.USDA.GOV)
Fri Oct 20 16:34:59 EST 2006

After focusing my proteins on an IEF gel, there are a number of clearly
visible white bands, especially at the "+" end.  I assume that this is
protein precipitating at it's isoelectric point.  How do I get these
back into solution to run the 2nd dimension?  Any tricks?


Rafael Garcia
Lead Scientist
Eastern Regional Research Center
600 East Mermaid Lane
Wyndmoor PA 19038

Rafael.Garcia from ars.usda.gov
voice: 215-836-3743
fax: 215-233-6795


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