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[Protein-analysis] Re: protein shape change detection

Geoff Briggs geoff.briggs at nottingham.ac.uk
Wed Sep 6 02:59:15 EST 2006


Carlo Zambonelli wrote:
> Hi,
> I'm trying to find out what is the best technique to detect protein shape
> change (i.e. globular to non-globular) in solution. I believe the only
> options are analytical ultracentrifugation or SEC-MALLS. I don't have
> experience with either technique and I would appreciate any feedback,
> reference, etc.
> Thank you very much.
> CZ


Have you considered using the standard biophysical techniques like CD 
and fluorescence? It sounds very much like you are doing something 
similar to a protein unfolding experiment, and many of the techniques 
that are used to study folding should be suitable for what you want.

Geoff


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