Carlo Zambonelli wrote:
> I'm trying to find out what is the best technique to detect protein shape
> change (i.e. globular to non-globular) in solution. I believe the only
> options are analytical ultracentrifugation or SEC-MALLS. I don't have
> experience with either technique and I would appreciate any feedback,
> reference, etc.
> Thank you very much.
Have you considered using the standard biophysical techniques like CD
and fluorescence? It sounds very much like you are doing something
similar to a protein unfolding experiment, and many of the techniques
that are used to study folding should be suitable for what you want.