In article <mailman.879.1157471559.20007.proteins at net.bio.net>,
"Carlo Zambonelli" <zambonel at gmail.com> wrote:
> I'm trying to find out what is the best technique to detect protein shape
> change (i.e. globular to non-globular) in solution. I believe the only
> options are analytical ultracentrifugation or SEC-MALLS. I don't have
> experience with either technique and I would appreciate any feedback,
> reference, etc.
> Thank you very much.
It would be best to find a collaborator who is expert in the area or
plan on spending a lot of time (the analysis is relatively simple but
since it involves model fitting it needs very good data and obtaining a
good data set will take a lot of time and effort). The developer of the
ULTRASCAN software package Bories Demeler at UT San Antonio has a couple
of 2-3 day courses on the software package etc. that is offered once or
twice a year. The other developers (Peter Schuck) offer similar courses
a few times a year though sometimes they are targeted at more advanced
users. If you cannot make it for one of these courses contacting them
may put you in contact with a suitable euro labs.