[Protein-analysis] Protein MW deviation

Sutk Nakasa kon_diew at hotmail.com
Sun Sep 17 23:59:32 EST 2006


Dear All,

why sometimes the target protein size is bigger than the expected size when 
running in SDS-PAGE? Some suggested that I should run my target protein 
compared with un-prestained protein markers, or to make sure that there is 
no cross-reaction once the immuboblot was performed. All of the above have 
been tested but the results with bigger size of target protein than that of 
expected one still a problem. Can anyone give me a clearer explaination or a 
better way to verify or to confirm that I have the right target protein?

Thank you very much,
Kon Diew

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