[Protein-analysis] How to express toxic proteins in bacteria - correction!

Tommy Sors tsors at purdue.edu
Thu Sep 21 13:48:49 EST 2006


Dear Dr. Gegenheimer,
I've read a post on the internet relating to the expression of toxic 
proteins in bacteria.  In this particular letter you wrote the following:

"A third method is to use a host cell lacking the T7 polymerase gene, and
induce target protein synthesis by infection with a lytic phage carrying the
T7 polymerase gene. The phage is available from Novagen and other sources."


If you have a chance, could you please let me know a bit more about this 
particular system.  For example, what strains lacking the T7 polymerase 
have worked well for you, and is there a protocol associated to this 
type of expression?  Moreover, I assume that there is no need for 
induction with IPTG since the induction would be enabled by the phage 
polymerase.  Does the expressed protein degrade in the media once the 
bacteria have undergone phage-specific lysis?

Thank you very much for your attention to this matter.

Best regards,
Tommy Sors
PhD. Candidate
Dept. of Horticulture
Purdue University


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