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[Protein-analysis] Re: How to express toxic proteins in bacteria - correction!

Duncan Clark blackhole at abuse.plus.com
Mon Sep 25 09:34:58 EST 2006


Historians believe that in newspost 
<mailman.1137.1158898190.20007.proteins at net.bio.net> on Thu, 21 Sep 
2006, Tommy Sors <tsors at purdue.edu> penned the following literary 
masterpiece:
>If you have a chance, could you please let me know a bit more about 
>this particular system.  For example, what strains lacking the T7 
>polymerase have worked well for you, and is there a protocol associated 
>to this type of expression?  Moreover, I assume that there is no need 
>for induction with IPTG since the induction would be enabled by the 
>phage polymerase.  Does the expressed protein degrade in the media once 
>the bacteria have undergone phage-specific lysis?

Novagen web site, Google, Pubmed for Studier etc.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.


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