[Protein-analysis] Re: Inferring rough protein structure from gel electrophoresis

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Sun Apr 15 14:04:19 EST 2007


Protenger wrote:

> Hi all
> Is there any body know how one can obtain protein structure
> information (roughly, for example hydrophobicity or roughly alpha or
> beta content and so on) by gel electrophoresis or related technics
> without using rigorous technics such as X-ray crystalography or NMR or
> prediction by bioinformatics tools.
> Or sudgest a book or review about that.

What you do get is the Stokes radius, which depends on size and shape of
the molecule. There are books out there on quantitative electrophoresis,
e.g. by Chrambach. IR-spectroscopy (amide bands) and far-UV circular
dichroism are techniques to determine approximate helix/sheet/turn/coil
ratios. 


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