[Protein-analysis] PLEASE HELP ........
kishor kumar keekan
(by kumars_kk from rediffmail.com)
Thu Aug 9 06:18:37 EST 2007
Dr. Peter Gegenheimer
Dept Biochem, Cell & Mol Biol; Dept Botany
University of Kansas
2045 Haworth Hall
Lawrence KS 66045-2106
Vox: 785-864-3939 FAX: 785-864-5321
This is in response to the answer from protocol online.
i am kishor doing PhD on Vanilla curing.
i wanted to extract Vanilla pod protein for PAGE,
Since the cured bean (dark brown in colour) contains large amount of polyphenols including all the flavour compounds.....etc the protein extract is dark brown in colour !!!
Please answer the following,
1 how can i extract protein for PAGE ?
2. What is the extraction media/Buffer ?
3 can i perform activity assay(PAGE) from the isolated protein, ? or how to isolate protein from the beans for activity assay ?
Please answer, to the the same e mail ID
it will be a great help,
kishor kumar k
dept of applied botany
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