[Protein-analysis] magnesium chloride in microsome buffer

Albert via proteins%40net.bio.net (by Neill.Horley from gmail.com)
Wed Dec 5 05:30:27 EST 2007


Hi
I use a NADPH regenerating system for analysis of my microsomal
enzymes. Does any one know if there is a need for EDTA in the original
microsomes preparation buffer? It seems odd to have EDTA in the
microsome preparation buffer and then to have magnesium chloride in
the NADPH regenerating system.
Any suggestions would be appreciated.


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