[Protein-analysis] frozen aliquots of protein:DNA complexes
Emily Arturo
via proteins%40net.bio.net
(by ecakbd At missouri.edu)
Thu Feb 1 14:00:04 EST 2007
Hello,
I have recently discovered that it will be to my great advantage that
I freeze (at -80degC) aliquots of my protein for use over long periods
of time. I want to do the same with the protein complexed with its
high-affinity DNA 'ligand'. Has anyone had experience with
successfully deep freezing protein:DNA complexes? fyi: 'successfully"
in my case would mean crystallizing the complex for x-ray defraction
studies.
Emily.
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