[Protein-analysis] Re: frozen aliquots of protein:DNA complexes

Emily Arturo via proteins%40net.bio.net (by ecakbd At missouri.edu)
Fri Feb 2 12:54:41 EST 2007


Great.  I will do it then.  Can you tell me what size of DNA were you
looking at?  Just for grins...

Emily.

> Message: 2
> Date: Fri, 02 Feb 2007 01:17:50 GMT
> From: dk At no.email.thankstospam.net (DK)
> Subject: [Protein-analysis] Re: frozen aliquots of protein:DNA
>         complexes
> To: proteins At net.bio.net
> Message-ID: <2jwwh.1291$833.1282 At newsfe05.lga>
>
> In article <mailman.1024.1170373661.19683.proteins At net.bio.net>, "Emily Arturo" <ecakbd At missouri.edu> wrote:
> >Hello,
> >
> >I have recently discovered that it will be to my great advantage that
> >I freeze (at -80degC) aliquots of my protein for use over long periods
> >of time.  I want to do the same with the protein complexed with its
> >high-affinity DNA 'ligand'.  Has anyone had experience with
> >successfully deep freezing protein:DNA complexes?  fyi: 'successfully"
> >in my case would mean crystallizing the complex for x-ray defraction
> >studies.
>
> Yep, at least two different complexes frozen at ~ 10 mg/ml protein
> as 30 ul drops into liquid nitrogen. Very handy for storage - you just
> roll out beads from screw cap tube stored at -80. Both complexes
> solved to 2.5A
>
> DK
>
>
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