[Protein-analysis] Re: Doing a western after coomassie staining

[Dr Engelbert Buxbaum]

Coomer wrote:

> Does anyone know a protocol to transfer protein after staining the gel
> with coomassie (10% Acetic acid and 50% EtOH, 0.2% Brilliant Blue) ?

Can be done with Dunns blotting buffer with 50 uM SDS added (or Towbins
buffer without methanol). Preincubate the gel with the buffer for 15-30
min to allow SDS-binding to the proteins. Works even if the gels had
been dryed, if they are re-hydrated in the buffer.

Note however that the yield will be lower than with unstained gels.