[Protein-analysis] Protein tagging

ANGELA LONDONO NAVAS via proteins%40net.bio.net (by londononavas At wisc.edu)
Thu Jan 4 16:59:52 EST 2007


Hi: 

I'm about to clone a gene into a pCMV-HA vector in order to express it in a mammalian cell line. I need to see the effect of that protein in the cell so i don't know if the presence of the tag won't validate my assay because it might be argued that i cannot prove it behaves as the actual native protein. 

In advance thak you for any help you can gice me. 

Angela L. 



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