[Protein-analysis] (no subject)

[Manisha Mishra]

hi

I have one problem when i do western blot..

Sometimes i get single bands but many times i get so many bands..while my
lab mates get few bands using the same antibody..What may be the probable
reason????

also i want to know whether wet method is good for tranfer or semidry????


-- 
Regards
Manisha Mishra

Manisha Mishra
Molecular biology Unit
Dr Klaus Heese
SBS, NTU
Singapore