[Protein-analysis] Re:1. (no subject) (Manisha Mishra)

Lu Falong via proteins%40net.bio.net (by FLLu At genetics.ac.cn)
Sun Jan 21 23:33:46 EST 2007


proteins-request,ÄúºÃ£¡

	 Usually, two many bands are caused by too much antibody you used. Tried to dilute more of your antibody when using. In my experience wet transfer has a much higher efficency than semi-dry tranfer. The only problem is buffer consuming and heat generating.

======= 2007-01-22 01:08:45 ÄúÔÚÀ´ÐÅÖÐдµÀ£º=======

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>Today's Topics:
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>   1. (no subject) (Manisha Mishra)
>   2. MicroCAL Origin 7.0 Software for ITC (Tyler Collins)
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>----------------------------------------------------------------------
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>Message: 1
>Date: Thu, 18 Jan 2007 11:45:04 +0800
>From: "Manisha Mishra" <manishamishra1 At gmail.com>
>Subject: [Protein-analysis] (no subject)
>To: Proteins At magpie.bio.indiana.edu
>Message-ID:
>	<ce5117a50701171945y5070f352q3aeb1dd467350167 At mail.gmail.com>
>Content-Type: text/plain; charset=ISO-8859-1; format=flowed
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>hi
>
>I have one problem when i do western blot..
>
>Sometimes i get single bands but many times i get so many bands..while my
>lab mates get few bands using the same antibody..What may be the probable
>reason????
>
>also i want to know whether wet method is good for tranfer or semidry????
>
>
>-- 
>Regards
>Manisha Mishra
>
>Manisha Mishra
>Molecular biology Unit
>Dr Klaus Heese
>SBS, NTU
>Singapore
>
>
>------------------------------
>
>Message: 2
>Date: Fri, 19 Jan 2007 16:51:27 -0500
>From: "Tyler Collins" <collinst824 At duq.edu>
>Subject: [Protein-analysis] MicroCAL Origin 7.0 Software for ITC
>To: <proteins At magpie.bio.indiana.edu>
>Message-ID: <ECEGJIFPMLAOOGGDGKODKENPCCAA.collinst824 At duq.edu>
>Content-Type: text/plain;	charset="iso-8859-1"
>
>I am attempting to use our ITC MCS Unit from MicroCal to quantify the
>homodimer dissociation of a dimeric protein to monomer.  Unfortunately,
>homodimeric species are difficult to analyze using the old Origin software.
>In addition, MicroCal will not provide a free upgrade in software to
>accommodate the models you want to use.  Instead, they are charging $2500
>for software that includes a dimer dissociation model that would be useful
>to my work.  Does anyone know where to get a possible bootleg version of the
>software, or do you have suggestions, books, articles that may aid in
>helping me write my own macro?
>
>
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Sincerely,¡¡¡¡¡¡¡¡¡¡

Lu Falong
 Group 808
 Institute of Genetics and Developmental Biology, CAS 
 Beijing, PR China
 FLLu At genetics.ac.cn
 2007-01-22  




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