[Protein-analysis] ghost" dots on blot

Ligong Wang via proteins%40net.bio.net (by ligongw2004 from yahoo.com)
Thu Jul 19 14:42:33 EST 2007


Did you block the membrane with BSA before you probe it with primary&secondary antibodies? If you did not, the primary Ab will bind to the membrane thus give higher background.

ligong

       
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