[Protein-analysis] Re: Glycerol during protein purification and protein oligomerization state

[Frank Küster]

"Dr Engelbert Buxbaum" <engelbert_buxbaum from hotmail.com> wrote:

> I would however suspect that removal of the DNA could be the culprit:
> many  proteins become unstable if their ligand is removed. Perhaps
> adding short  DNA fragments, which are less likely to interfere with
> purification than  an entire bacterial chromosome, would help.

Or even just high concentrations (100 or 200 mM) of phosphate buffer.  I
know about an (unpublished) DNA-binding protein which can be triggered
to fold in a stopped-flow by mixing protein solution with  phosphate
buffer. 

Regards, Frank

-- 
(17:00:03) ***joeyh loves that install-info uses $'
(17:00:34) Yoe: what's $' again?
(17:00:49) joeyh: shorthand for make your perl program slow at the 
                  expense of readability