[Protein-analysis] Deglycosylation of N Oligosaccharides
(by crichard from su.edu)
Tue Jul 31 12:56:15 EST 2007
Checking to see if you are available for some endoglycosidase questions.
I've removed different Asn-linked carbohydrate chains from an intact
glycoprotein (non denatured, non reduced, non heated), but when I run it
on a non-reducing gel, some degly forms are the same MW as the non-degly
control, or are a larger MW than the degly control. Reducing gels give
clear confirmation of deglycosylation.
Share any thoughts if you like.
Craig A. H. Richard Ph.D.
Assistant Professor, Biopharmaceutical Sciences
Bernard J. Dunn School of Pharmacy, Shenandoah University
1775 North Sector Court, Winchester, VA 22601
Phone: 540-678-4387 Fax: 540-665-1283
Email: crichard from su.edu <mailto:crichard from su.edu>
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