[Protein-analysis] Deglycosylation of N Oligosaccharides

Richard, Craig. via proteins%40net.bio.net (by crichard from su.edu)
Tue Jul 31 12:56:15 EST 2007



Checking to see if you are available for some endoglycosidase questions.


I've removed different Asn-linked carbohydrate chains from an intact
glycoprotein (non denatured, non reduced, non heated), but when I run it
on a non-reducing gel, some degly forms are the same MW as the non-degly
control, or are a larger MW than the degly control.  Reducing gels give
clear confirmation of deglycosylation.


Share any thoughts if you like.


Thanks, Craig


Craig A. H. Richard Ph.D.

Assistant Professor, Biopharmaceutical Sciences

Bernard J. Dunn School of Pharmacy, Shenandoah University

1775 North Sector Court, Winchester, VA 22601

Phone: 540-678-4387  Fax: 540-665-1283  

Email: crichard from su.edu <mailto:crichard from su.edu> 




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