[Protein-analysis] Re: Reduction of Cu (II) in protein assays

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Fri Jun 1 13:04:17 EST 2007


Am 24.05.2007, 06:39 Uhr, schrieb Joana Arroz <joanaarroz from isa.utl.pt>:

> untill now i thougth that quantify proteins was not a major problem!!!.

Think never to have thought, for the thinking of thoughts is thoughtless  
thinking!

Indeed, there is no method for absolute protein determination that could  
be performed at the sample size required in a modern enzymological lab.  
Absolute means that you do not need reference standards. Also, many  
commonly used substances (detergents, reducing agens, complex formers,  
urea) interfere with many protein assays.

So in a word: the situation is hopeless, but not serious.


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