[Protein-analysis] Re: Reduction of Cu (II) in protein assays
Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Fri Jun 1 13:04:17 EST 2007
Am 24.05.2007, 06:39 Uhr, schrieb Joana Arroz <joanaarroz from isa.utl.pt>:
> untill now i thougth that quantify proteins was not a major problem!!!.
Think never to have thought, for the thinking of thoughts is thoughtless
Indeed, there is no method for absolute protein determination that could
be performed at the sample size required in a modern enzymological lab.
Absolute means that you do not need reference standards. Also, many
commonly used substances (detergents, reducing agens, complex formers,
urea) interfere with many protein assays.
So in a word: the situation is hopeless, but not serious.
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