[Protein-analysis] Re: Reduction of Cu (II) in protein assays

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Thu Jun 14 11:35:16 EST 2007


Am 01.06.2007, 14:54 Uhr, schrieb DK <dk from no.email.thankstospam.net>:

> What about total amino acid analysis? I think some implementations
> of it are direct, requiring no standards.

But it is laborious, time consuming and requires relatively large sample  
sizes. Seal samples with 6 N HCl in vacuo, heat o/n, open the ampulles, do  
quantitative HPLC. And with that many steps I'd rather not know what the  
reproducibility would be.

Although, admittedly, one could use alkaline hydrolysis instead of HCL  
which is slightly easier.


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