[Protein-analysis] Re: one-to-one protein-to-DNA binding

Clement Angkawidjaja via proteins%40net.bio.net (by clement from bio.mls.eng.osaka-u.ac.jp)
Wed Mar 28 00:45:36 EST 2007


Hi Emily,

Some direct methods are available to study the kinetics of protein to DNA 
binding.

1. Isothermal titration calorimetry.
2. Surface plasmon resonance.

Both are widely used, but the second is easier in practice, to my opinion. 
You need a tag (His-tag will do) on your protein for it to be immobilized. 
You can measure kinetics very sensitively and quickly using these two 
techniques.

Regards,
Clement 



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