[Protein-analysis] Biotechnology Progress manuscript # bp070155b

Biotechnology Progress via proteins%40net.bio.net (by biotech-prog from engr.ucr.edu)
Mon May 21 22:00:54 EST 2007


We are seeking reviewers/referees for the manuscripts submitted to our
journal for publication. 

 

 It has been very difficult identifying referees for this manuscript. If it
is possible for you to help us out, we ask that you please do. We need
referees for the following manuscript:

 

(20 pages) "Use of a derivative of Escherichia coli BL21(DE3) for efficient
productions of three different recombinant proteins", by Jing-Bo Zhao,
Dong-Zhi Wei

Here is the abstract:


------------------------------


Plasmid instability and growth inhibition of plasmid-bearing cells after
induction were encountered when E. coli BL21(DE3) was used as host for the
production of anti-human ovarian carcinoma ? anti-human CD3 single chain
bispecific antibody (AhOC?AhCD3), human soluble B lymphocyte stimulator
fused with thioredoxin (Trx-hsBLyS) and human parathyroid hormone fused with
thioredoxin (Trx-hPTH), respectively. A derivative of BL21(DE3), namely
BLRM(DE3), isolated and showing superiority in AhOC?AhCD3 production in our
previous work, was further used here for more efficient production of the
three different recombinant proteins. By using BLRM(DE3) as host, the
simplified one-stage fermentation process was developed, which was more
laborsaving and yielded AhOC?AhCD3 comparable to that of the traditional
two-stage fermentation process. Also, the plasmid stabilities and production
yields of Trx-hsBLyS and Trx-hPTH were dramatically improved by the
application of BLRM(DE3) instead of BL21(DE3). A high Trx-hsBLyS yield
(about 3.5 g/L) was obtained, which was more than twice as much as that of
the recombinant BL21(DE3) strain. The Trx-hPTH yield was improved from about
700 mg/L to 1 g/L. These results further showed the superiority of BLRM(DE3)
to BL21(DE3) and suggested its effectiveness for other BL21(DE3)/pET
heterologous protein expression systems which encounter similar problems.

 -----------------------------

 

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Sincerely, 

Ivy Hughes

___________________________

 

 

Ivy Hughes

Assistant Editor

Biotechnology Progress

Bourns College of Engineering

University of California, Riverside

Bourns Hall A220

Riverside, CA 92521-0001

 

Tel: (951) 827-7977

Fax: (951) 827-7978

Email: biotech-prog from engr.ucr.edu

 

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Biotechnology Progress manuscript # bp070155b

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 



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