[Protein-analysis] Determination of Triton X114 concentration

Jyothi Jayaram via proteins%40net.bio.net (by jyothi.jayaram from gmail.com)
Mon Sep 17 15:34:17 EST 2007


Hi there,

I need help in determining the concentration of the detergent Triton X-114
in water saturated Triton 114. I know that once the detergent is water
saturated, we have to determine the absorbance at 277 nm. After this, I
really don't know how to correlate the value obtained to its concentration.
Any help in this matter is appreciated.

Thanks,
Jyothi


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