[Protein-analysis] Re: Determination of Triton X114 concentration

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Thu Sep 20 09:35:56 EST 2007


Am 17.09.2007, 16:34 Uhr, schrieb Jyothi Jayaram  
<jyothi.jayaram from gmail.com>:

> Hi there,
>
> I need help in determining the concentration of the detergent Triton  
> X-114
> in water saturated Triton 114. I know that once the detergent is water
> saturated, we have to determine the absorbance at 277 nm. After this, I
> really don't know how to correlate the value obtained to its  
> concentration.

See Nag et al., Anal. Biochem. 237 (1996) 224-231 and Garewal Anal.  
Biochem. 54 (1973) 319-324. The procedure works with all poly-oxethylen  
detergents.


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