[Protein-analysis] DNA binding protein purification

Itisam Sarangi via proteins%40net.bio.net (by itisam.sarangi from gmail.com)
Mon Apr 28 12:16:01 EST 2008


Dear all
Presently I am trying to purifying a DNA binding protein from E.coli..and
trying to remove the contaminating DNA...
I am using DNAse to remove the contaminating DNA ....but the protein is
precipitaing in the contact with diavalent cations used for DNAse
activity...if any one have  suggestions plz feel free to share....


itisam


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