[Protein-analysis] Re: Protocol needed from study the protein aggregation

[Clement Angkawidjaja]

> Hi all
> Recently i got a mutant gene and showing higher solubility then wild
> type. we are assuming that our mutant preventing the aggregation of the
> protein.
> I would like to study the thermal and chemical protein aggregation.
> i would be happy If any one provide me detailed protocol to study this
> experiment.

Besides DSC, you can also do CD spectroscopy. Easier and needs smaller 
amount of protein (10 mg for far-UV should be sufficient). Similar with Dr. 
Engelbert Buxbaum's comment, you need to collaborate with someone with 
appropriate instrument.

Clement