[Protein-analysis] Recombinant Protein precipitate out under 20 mM KPB/100 mM NaCl ???

Nico via proteins%40net.bio.net (by brighttomorrow from gmail.com)
Thu Feb 14 19:21:21 EST 2008


Hi, 

I am changing buffer for my protein, which was purified from Ni-NTA column.
Under 20mM KPB/500mM NaCl, it was good. But when change to 20 mM KPB/100 mM NaCl,
the protein precipitate out. What should I do? Change to Tris-HCl Buffer or.....

Please help me out,

Many thanks,

Nico




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