[Protein-analysis] Two bands on p53 WB.

Alejandro Sanchez via proteins%40net.bio.net (by asanchez from bio.puc.cl)
Thu Jan 10 15:07:12 EST 2008


Hello
I´m performing western blots on lysates from EBV transformed lymphocytes. I 
electrophorese the lysates on a 12% SDS PAGE and the incubation with the 
anti p53 DO1 shows a double band in some individuals and only one band in 
others, the difference between the bands is approx 3-4 kDa. This happens 
without any treatment. Some one has experienced this???. Is this due to 
phosphorylation???, p53 isoforms differentially expressed in different 
individuals???.
I´ll appreciate any suggestion,
Alejandro





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