[Protein-analysis] Re: His-tagged protein purification from yeast
(k lactis) supernatant
Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Wed Jan 16 13:48:09 EST 2008
Am 04.01.2008, 16:03 Uhr, schrieb DK <dk from no.email.thankstospam.net>:
> Well, that means you need to get rid of the medium and all of my
> suggestions are potentially applicable.
Yes, I think so too. I'd probably try to concentrate my extract in an
Amicon pressure cell, add a suitable buffer and repeat a couple of times.
That should remove most of the low MW contaminants.
Even better of course to pellet the cells in a centrifuge, resuspend and
then lyse in a defined medium. I never liked the idea of proprietary
reagents where you don't know what the hell you are getting into.
More information about the Proteins