[Protein-analysis] Re: protein extraction

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Wed Jan 16 14:25:56 EST 2008


Am 14.01.2008, 10:17 Uhr, schrieb kyoli <allicindixvin from gmail.com>:

> i am performing 1-D gel electrophoresis for protein  from leave but
> chlorophyll is inhibiting for which i used acetone with tris. do
> anyone have any method to extract protein in this combination without
> using liquid nitrogen

Chloroform/Methanol precipitation should work

@article{Wes-84,
         AUTHOR= {D. Wessel and U.I. Flügge},
         TITLE= {A Method for the quantitative recovery of protein in the  
presence of detergents and lipids},
         JOURNAL= {Anal. Biochem.},
         VOLUME= {138},
         YEAR= {1984},
         PAGES= {141-143},
         LANGUAGE= {engl}
}


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