[Protein-analysis] preparing proteins for lyphilization

jps via proteins%40net.bio.net (by sumida from bbri.org)
Fri Jul 4 02:30:12 EST 2008


I think it is common practice to dialyze proteins in an ammonium
bicarbonate solution at pH=8.  Presumably this is a way to maintain
the protein with some salt to stabilize it while in solution, but not
have any salt present after this protein solution is lyophilized since
the ammonia sublimates and carbon dioxide is released during freeze
drying step.

However, I am wondering if this is actually accurate.  Can anybody
comment on this procedure?


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