[Protein-analysis] NEM-S1

jps via proteins%40net.bio.net (by sumida from bbri.org)
Fri Jul 11 21:11:37 EST 2008


N-ethylmaleimide modification of the SH1 and SH2 groups appears to be
a frequently used method to convert the myosin S1 subunit into a
strong-binding but inactive (ATPase-wise) form of the S1.  This seems
to have been a useful tool to investigate the regulatory properties of
the muscle thin filament activation and cooperativity.

Can anyone comment on the modification process? Is the actin/NEM-S1
binding constant well characterized?  Is it always the same?  Does NEM-
S1 precipitate out of solution over time?

Thank you.


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