[Protein-analysis] Freeze Dryer vs Speed Vac

Dorit Grunberger via proteins%40net.bio.net (by dorit.grunberger from radiology.ucsf.edu)
Mon Jul 14 20:33:40 EST 2008


Saw this online and thought to ask you if it makes sense to hook up a
speedvac to a lyophilizer both as source of vacuum and no less important, a
cold environment to keep my extract happy. I¹m doing chloroform : methanol :
water extracts of small tissue samples. The total volumes for extraction
will be small and I was hoping to use a speedvac to dry/concentrate my
MeOH/H2O phase. It¹s critical for the sample to remain as cold as possible,
since the analysis of contents is done by NMR and we¹re hoping to maintain
integrity of some very easily degradable metabolites (phospho-choline for
example).

Bottom line: do you think this will work?

Thanks in advance for any help.

Dorit Grunberger
Department of Radiology
email:  dorit.grunberger from radiology.ucsf.edu
415.514.4845   office
415.385.0977  cell
415.514.2550  fax





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