[Protein-analysis] Re: Freeze Dryer vs Speed Vac

allisonh via proteins%40net.bio.net (by allison from nospam.com)
Wed Jul 16 11:19:23 EST 2008


If a cold temperature is important maybe you could just run the Speedvac 
in a cold room (with the heater turned off of course).
Allison

Dorit Grunberger wrote:
> Saw this online and thought to ask you if it makes sense to hook up a
> speedvac to a lyophilizer both as source of vacuum and no less important, a
> cold environment to keep my extract happy. I¹m doing chloroform : methanol :
> water extracts of small tissue samples. The total volumes for extraction
> will be small and I was hoping to use a speedvac to dry/concentrate my
> MeOH/H2O phase. It¹s critical for the sample to remain as cold as possible,
> since the analysis of contents is done by NMR and we¹re hoping to maintain
> integrity of some very easily degradable metabolites (phospho-choline for
> example).
> 
> Bottom line: do you think this will work?
> 
> Thanks in advance for any help.
> 
> Dorit Grunberger
> Department of Radiology
> email:  dorit.grunberger from radiology.ucsf.edu
> 415.514.4845   office
> 415.385.0977  cell
> 415.514.2550  fax
> 
> 
> 


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