[Protein-analysis] Re: Freeze Dryer vs Speed Vac
(by sumida from bbri.org)
Thu Jul 17 09:12:58 EST 2008
On Jul 16, 12:19 pm, allisonh <alli... from nospam.com> wrote:
> If a cold temperature is important maybe you could just run the Speedvac
> in a cold room (with the heater turned off of course).
> Dorit Grunberger wrote:
> > Saw this online and thought to ask you if it makes sense to hook up a
> > speedvac to a lyophilizer both as source of vacuum and no less important, a
> > cold environment to keep my extract happy. I¹m doing chloroform : methanol :
> > water extracts of small tissue samples. The total volumes for extraction
> > will be small and I was hoping to use a speedvac to dry/concentrate my
> > MeOH/H2O phase. It¹s critical for the sample to remain as cold as possible,
> > since the analysis of contents is done by NMR and we¹re hoping to maintain
> > integrity of some very easily degradable metabolites (phospho-choline for
> > example).
> > Bottom line: do you think this will work?
> > Thanks in advance for any help.
> > Dorit Grunberger
> > Department of Radiology
> > email: dorit.grunber... from radiology.ucsf.edu
> > 415.514.4845 office
> > 415.385.0977 cell
> > 415.514.2550 fax- Hide quoted text -
> - Show quoted text -
Here's another question along these lines: canthe lyophylization
process itself does not denature your protein?
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