[Protein-analysis] Re: Proteins Digest, Vol 36, Issue 1

Jyothi Jayaram via proteins%40net.bio.net (by jyothi.jayaram from gmail.com)
Thu May 1 13:44:42 EST 2008


Thank you!
J

On Thu, May 1, 2008 at 1:03 PM, <proteins-request from oat.bio.indiana.edu>
wrote:

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>   1. Re: Protocol needed from study the protein        aggregation
>      (Clement Angkawidjaja)
>
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> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 30 Apr 2008 09:20:11 +0900
> From: "Clement Angkawidjaja" <clement from bio.mls.eng.osaka-u.ac.jp>
> Subject: [Protein-analysis] Re: Protocol needed from study the protein
>        aggregation
> To: <proteins from oat.bio.indiana.edu>
> Message-ID: <003101c8aa57$f46a1a10$0e00a8c0 from CLEMENT>
> Content-Type: text/plain; format=flowed; charset="ISO-8859-1";
>        reply-type=original
>
> > Hi all
> > Recently i got a mutant gene and showing higher solubility then wild
> > type. we are assuming that our mutant preventing the aggregation of the
> > protein.
> > I would like to study the thermal and chemical protein aggregation.
> > i would be happy If any one provide me detailed protocol to study this
> > experiment.
>
> Besides DSC, you can also do CD spectroscopy. Easier and needs smaller
> amount of protein (10 mg for far-UV should be sufficient). Similar with
> Dr.
> Engelbert Buxbaum's comment, you need to collaborate with someone with
> appropriate instrument.
>
> Clement
>
>
>
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> End of Proteins Digest, Vol 36, Issue 1
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-- 
Jyothi Jayaram Vishnumangalam,
8 Country Club Rd, # 31
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