[Protein-analysis] RE: Proteins Digest, Vol 41, Issue 1

maria.holmberg from risoe.dk via proteins%40net.bio.net (by maria.holmberg from risoe.dk)
Thu Oct 2 00:56:08 EST 2008


If I understand your question right it is just a question of
transferring mCi/g to mCi/mmol... 
The equation states that the amount in moles is the amount in grams
divided by the molecular weight of the species (n=m/M).
Thus 200 mCi/g = 200 mCi/(1g/463.6 g/mol) = 200 mCi/(1/463.6)mol 

Regards Maria

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Today's Topics:

   1. Help with calculations for specific activity  (Fasulo, Lisa M)


----------------------------------------------------------------------

Message: 1
Date: Tue, 30 Sep 2008 16:55:26 -0400
From: "Fasulo, Lisa M" <lisa.m.fasulo from pfizer.com>
Subject: [Protein-analysis] Help with calculations for specific
	activity 
To: <proteins from magpie.bio.indiana.edu>
Message-ID:
	
<3F4063B77CC1F445933078B93A0A0C720855F3CF from groamrexm05.amer.pfizer.com>
Content-Type: text/plain;	charset="us-ascii"

I am trying to calculate the specific activity of my [3H]-ligand but
don't know how to do it.  The package insert for my radioligand states
that the Specific Activity is 200 mCi/g but I need to know what the
Specific Activity is in Ci/mmol.  How do I calculate this?  The
molecular weight of the radioligand is 463.6 g/mol.  Can anyone walk me
through the calculations to give the specific activity in Ci/mmol?  The
only other info I have is the concentration of the radioligand which is
1mCi/mL.

Lisa



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