[Protein-analysis] gas chromatography

kyoli via proteins%40net.bio.net (by allicindixvin from gmail.com)
Tue Sep 2 07:42:47 EST 2008


hello to all,
 i am new in the field and trying to run my sample in GC. i have
extracted my oil from seed in petroleum ether using soxhlet apparatus.
to transesterifying it it is treated with methanolic KOH. the problem
starts now as i don't know that how to prepare the standard sample. i
have standards of linoleic acid and oleic acid from sigma aldrich. i
don't know the exact procedure to prepare these standards so as to run
into GC. can it be made by simply dissolving in methanol


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