[Protein-analysis] hydroxyapatite columns problems
(by nicolas.bazeille from curie.u-psud.fr)
Mon Feb 2 11:43:50 EST 2009
I'm trying to purify protein using hydroxypapatite system from bio-rad. The
purification step work well, but the powder is very hard to regenerated.
I don't use any forbidden components in my buffer such EDTA or Tris.
I use different kinds of regeneration pathways (500 mM phosphate, 1M NaOH,
6M guanidium chloride for short time) according to the supplier
informations. However, after regeneration, the flow rate greatly decrease,
and the powder seems to be damaged (it seems to be solubilize while
solution containing phosphate). I don't want to use new product for each
purification Is there a way to preserve the stability of the material ?
Is somebody find the same problem?
thanks in advance.
M. BAZEILLE Nicolas
Génotoxicologie et cycle cellulaire - UMR 2027 CNRS/Institut Curie
91405 ORSAY Cedex
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