[Protein-analysis] proteins that don't ionize

jps via proteins%40net.bio.net (by sumida from bbri.org)
Fri Feb 20 12:46:17 EST 2009


Recently purified an unknown protein with a partial N-terminal
sequence match to a bacterial outermembrane protein.  Oddly this
protein does not react with DTNB, does not seem to ionize when mixed
with sinnapinic acid and placed in a MALDI-ToF, and has a molecular
weight of 33kDa as judged by SDS PAGE analysis.

Curious to know if anybody thinks that membrane proteins would have
more difficulty interacting with MALDI matrices than other proteins?


More information about the Proteins mailing list