[Protein-analysis] Re: proteins that don't ionize
(by piejem from hotmail.com)
Wed Mar 4 06:49:46 EST 2009
> Recently purified an unknown protein with a partial N-terminal
> sequence match to a bacterial outermembrane protein. Oddly this
> protein does not react with DTNB, does not seem to ionize when mixed
> with sinnapinic acid and placed in a MALDI-ToF, and has a molecular
> weight of 33kDa as judged by SDS PAGE analysis.
> Curious to know if anybody thinks that membrane proteins would have
> more difficulty interacting with MALDI matrices than other proteins?
Maybe trie a batch of matrices?
Not all substrates wil "fly" with sinnapinic acid. DHBA? maybe or use
electrospray (TOF) MS.
More information about the Proteins