[Protein-analysis] sca-1

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Mon Mar 23 07:10:18 EST 2009


Am 16.03.2009, 17:37 Uhr, schrieb mmazan01 <melissa.mazan from tufts.edu>:

>
> Hi - I am trying to detect Ly6 A/E (sca-1) from mouse lung on western  
> blot.
> I am using a monoclonal rat anti-mouse from ebiosciences.  I have very  
> clean
> bands, no background, but at 75 kd instead of the expected 18 kd.  Can
> anyone shed light on this? Melissa

Sounds like you are looking at a tetramer. 18 kDa x 4 = 72 kDa, the  
difference is within the expected error margin. Did you heat the sample  
with DTT and SDS? What does the manufacturer of the antibody and the  
literature say about what you should find?


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