[Protein-analysis] Re: zinc finger protein purification issue

Dr Engelbert Buxbaum via proteins%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Mon Jan 25 16:39:20 EST 2010


Am 15.01.2010, 02:33 Uhr, schrieb 김종문 <jin.zongwen from gmail.com>:

> I recently started to purify a protein containing zinc finger modif.
>
> the protein consist of flexible linker and his tag in the N term and C
> terminal of the mofit.
>
> the Zinc finger itself is consisted from three zinc finger motifs.
>
> It expressed well in the E.coli, and I was able to purify the protein,  
> but
> when I tried to concentrate it by using a membrane filter( amicon  
> centricon)
> the protein start to aggregate.

"flexible linker" may mean "intrinsically disordered", which would explain  
the behaviour. However, without knowning more about that domain, I can  
only guess.


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