giardia and cryptosporidium

ACHAMPIO at RNA.BIO.MQ.EDU.AU ACHAMPIO at RNA.BIO.MQ.EDU.AU
Tue Aug 16 19:36:43 EST 1994


>From:          JarmeySwan at bchm.unp.ac.za (Claire.Jarmey(c)Swan)
>Subject:       giardia and cryptosporidium
>Date:          Thu, 11 Aug 1994 08:05:39 GMT
>
>>I am interested in methods of contrating, detecting these 
protozoa. 
>Also any 
>statistics on cases due to giardia  and cryptosporidium
>

	Is your interest in concentrating and analysing infected faecal 
samples or environmental samples?.  Generally identification from 
faecal samples is not too much of a problem, and I see other people 
have responded with refs for this.  The problem with environmental 
detection of these organisms is that they occur in very low numbers 
in the environment (which can still be a significant health problem) 
and therefore large volumes of water have to be analysed.We 
routinely analyse environmental water samples for Crytposporidium 
and Giardia by flow cytometry, using flocculation to concentrate 
samples.  We can easily concentrate 10litre samples by this method, 
and find the flow cytometry detection method very efficient.  I have 
given references below for our methods.  Other methods which are in 
general use are concentration by filtration, and analysis by 
epifluorescent microscopy.  References for these methods may be 
found in the papers given below.  There are several immunodiagnostic 
kits around which work well for both diagnosis in feacal samples and 
for detection in water.  The flow cytometry method requires cell 
sorting and verification by microscopy, but samples are much easier 
to interpret after sorting.  Please let us know if you have trouble 
getting hold of these references, and we will send them.  
				Yours,
				Alan Champion, Graham Vesey,
				Australian Environmental Flow Cytometry Group
				MUCAB, School of Biological Sciences
				Macquarie University.

Refs: 	Vesey, G., Slade, J. S., Byrne, M., Shepard, K. and Fricker, 
C. R. 1993a. A 	new method for the concentration of Cryptosporidium 
oocysts from water. J. 	Appl. Bacteriol. 75:82-86.
	Vesey, G., Slade, J. S., Byrne, M., Shepard, K. Dennis, P. J., and 
Fricker, 	C. R. 1993b. Routine monitoring of Cryptosporidium oocysts 
in water using 	flow cytometry. J. Appl. Bacteriol. 75:87-90.
	Vesey, G., Hutton, P. E., Champion, A. C., Ashbolt, N. J., 
Williams, K. L., 	Warton, A., and Veal, D. A. 1994. Application of 
flow cytometric methods 	for the routine detection of 
Cryptosporidium  and Giardia in water. 	Cytometry, 16:1-6.

Alan Champion,
School of Biological Sciences,
Macquarie University,
Sydney, Australia, 2109

Phone:(02) 850-8219
Fax: (02) 850-8174



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