DNA for zoo blot
Thomas Cosgrove Marsh
cosmo at iastate.edu
Tue Oct 25 13:14:03 EST 1994
In <38hipg$3iu at lynx.unm.edu> ebraun at triton.unm.edu (Edward Braun) writes:
>I am a graduate student at the University of New Mexico and I am
>currently trying to put together a zoo blot. I would really like to
>put togeter a blot that covers a broad range of taxa (I am interested
>in a protein that is present in (eu)bacteria, archaea, and in
>eukaryotes) and really wanted to get DNA from at least a few protista
>spp., since I wanted to look at the diversity that is available in this
>I am thinking right now that it would be appropriate to get DNA from
>Giardia lamblia, a flagellate (I was thinking Euglena or a
>Trypanosome), and a ciliate (Tetrahymena perhaps, or Paramecium). If
>anybody has "extra" DNA available (or cells - I'm happy to do the prep.
>myself) pls contact me by e-mail (ebraun at triton.unm.edu). My primary
>focus is yeast genetics, so the lab I work in is not set up to grow a
>wide variety of organisms, but even cells of something that is
>reasonable to grow (and isn't pathogenic!-I envision infecting myself)
>would be appreaciated.
>Thanks in advance.
>Edward Braun (ebraun at triton.unm.edu)
I donot have any Tetrahymena genomic DNA but many of my
labmates do. E-mail Shawn Ahmed (rush at iastate.edu). He may have some
to spare or may know who else could provide you with some.
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