DNA for zoo blot

Thomas Cosgrove Marsh cosmo at iastate.edu
Tue Oct 25 13:14:03 EST 1994


In <38hipg$3iu at lynx.unm.edu> ebraun at triton.unm.edu (Edward Braun) writes:

>I am a graduate student at the University of New Mexico and I am
>currently trying to put together a zoo blot.  I would really like to
>put togeter a blot that covers a broad range of taxa (I am interested
>in a protein that is present in (eu)bacteria, archaea, and in
>eukaryotes) and really wanted to get DNA from at least a few protista
>spp., since I wanted to look at the diversity that is available in this
>group.

>I am thinking right now that it would be appropriate to get DNA from
>Giardia lamblia, a flagellate (I was thinking Euglena or a
>Trypanosome), and a ciliate (Tetrahymena perhaps, or Paramecium).  If
>anybody has "extra" DNA available (or cells - I'm happy to do the prep.
>myself) pls contact me by e-mail (ebraun at triton.unm.edu).  My primary
>focus is yeast genetics, so the lab I work in is not set up to grow a
>wide variety of organisms, but even cells of something that is
>reasonable to grow (and isn't pathogenic!-I envision infecting myself)
>would be appreaciated.

>Thanks in advance.

>Edward Braun (ebraun at triton.unm.edu)

Greetings,
	I donot have any Tetrahymena genomic DNA but many of my
labmates do.  E-mail Shawn Ahmed (rush at iastate.edu).  He may have some
to spare or may know who else could provide you with some.


Good luck
cosmo

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