Dpt. of Cell Morphology Cell.Morphology at
Thu Sep 12 11:40:54 EST 1996

Dear Nathaniel,

we have no experience in labelling TRICHOMONAD nuclei, but for similar 
purposes we use Hoechst Dye (Bisbenzimid), purchased from Ridel-de-Haen 
in Hannover, FRG. As vital stain it is applied as 10mikroM solution for 
several hours, on fixed cells we use 100mikroM for 10 min. and rinse 
I can't promise, that this works with your cells, it's just a suggestion.

Ralf Breuker, at the
Department of Cell Morphology
NDEF 05 / Ruhr University Bochum
44780 Bochum, FRG

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