How to get rid of autofluorescence?

Rene Groben rgroben at awi-bremerhaven.de
Fri Aug 6 08:25:31 EST 1999


Dear collegues,

I am working on the identification of unicellular algae with fluorescent
probes. The problem I have is the sometimes strong autofluorescence of 
chlorophyll that irradiates over my probe signals in the epifluorescence 
microscope. Has anyone of you an idea of how to get rid of chlorophyll 
(chemicals, treatment)? So far I tried Methanol/Ethanol treatment.

If possible send me your answers by e-mail. Thank you for your help!

Rene Groben
-- 
Dr. Rene Groben
Alfred Wegener Institute for Polar and Marine Research
BIO II
Am Handelshafen 12
27570 Bremerhaven
Germany

Tel.: +49 (0)471/4831456
Fax.: +49 (0)471/4831425
e-Mail: rgroben at awi-bremerhaven.de



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